esr2 antagonist Search Results


or a selective esr2 agonist dpn ( )  (Cayman Chemical)
90
Cayman Chemical or a selective esr2 agonist dpn ( )
Gonadotropin stimulation resulted in an increased number of antral follicles in <t>Esr2-/-</t> ovaries. Wildtype (WT) and Esr2-/- rats were treated with exogenous gonadotropins on PND28. 48h after PMSG (30IU) injection, rats were administered with hCG (30IU). 4h after the hCG injection, ovaries were collected and processed for histological examination or collection of COCs. Histological examination demonstrated the presence of ovarian follicles at different stages of development in WT rats ( A ). In contrast, Esr2-/- rat ovaries showed an increased number of antral follicles ( B ). Cumulus cells were detached from the oocytes by mechanical pipetting before counting under microscope. Oocyte yield was about three-fold higher in Esr2-/- rat ovaries ( C ). Serially sectioned whole ovaries were stained with H&E, and follicles at different stages were counted in WT and Esr2-/- rats ( D ). Follicle counting demonstrated a decreased number of primordial follicles and an increased number of activated follicles in Esr2-/- rats ( E-G ). Data shown as mean ± SE, n≥3. * P ≤ 0.05.
Or A Selective Esr2 Agonist Dpn ( ), supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/esr2+antagonist/bio_rxiv__2020__02__06__937953-52-9-15?v=Cayman+Chemical
Average 90 stars, based on 1 article reviews
or a selective esr2 agonist dpn ( ) - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier

Image Search Results


Gonadotropin stimulation resulted in an increased number of antral follicles in Esr2-/- ovaries. Wildtype (WT) and Esr2-/- rats were treated with exogenous gonadotropins on PND28. 48h after PMSG (30IU) injection, rats were administered with hCG (30IU). 4h after the hCG injection, ovaries were collected and processed for histological examination or collection of COCs. Histological examination demonstrated the presence of ovarian follicles at different stages of development in WT rats ( A ). In contrast, Esr2-/- rat ovaries showed an increased number of antral follicles ( B ). Cumulus cells were detached from the oocytes by mechanical pipetting before counting under microscope. Oocyte yield was about three-fold higher in Esr2-/- rat ovaries ( C ). Serially sectioned whole ovaries were stained with H&E, and follicles at different stages were counted in WT and Esr2-/- rats ( D ). Follicle counting demonstrated a decreased number of primordial follicles and an increased number of activated follicles in Esr2-/- rats ( E-G ). Data shown as mean ± SE, n≥3. * P ≤ 0.05.

Journal: bioRxiv

Article Title: A gatekeeping role of ESR2 to maintain the primordial follicle reserve

doi: 10.1101/2020.02.06.937953

Figure Lengend Snippet: Gonadotropin stimulation resulted in an increased number of antral follicles in Esr2-/- ovaries. Wildtype (WT) and Esr2-/- rats were treated with exogenous gonadotropins on PND28. 48h after PMSG (30IU) injection, rats were administered with hCG (30IU). 4h after the hCG injection, ovaries were collected and processed for histological examination or collection of COCs. Histological examination demonstrated the presence of ovarian follicles at different stages of development in WT rats ( A ). In contrast, Esr2-/- rat ovaries showed an increased number of antral follicles ( B ). Cumulus cells were detached from the oocytes by mechanical pipetting before counting under microscope. Oocyte yield was about three-fold higher in Esr2-/- rat ovaries ( C ). Serially sectioned whole ovaries were stained with H&E, and follicles at different stages were counted in WT and Esr2-/- rats ( D ). Follicle counting demonstrated a decreased number of primordial follicles and an increased number of activated follicles in Esr2-/- rats ( E-G ). Data shown as mean ± SE, n≥3. * P ≤ 0.05.

Article Snippet: A selective ESR2 antagonist PHTPP ( ) or a selective ESR2 agonist DPN ( ) (Cayman Chemical, Ann Arbor, MI) was dissolved in DMSO (5µg/µl).

Techniques: Injection, Microscopy, Staining

Increased activation of primordial follicles in Esr2-/- rat ovaries. Follicle counting in PND28 ( A-C, J, M ), PND16 ( D-F, K, N ), and PND8 ( G-I, L, O ) wildtype (WT) and Esr2-/- rats showed increased activation of primordial follicles in Esr2-/- ovaries. Primordial follicle activation was near two-fold starting on PND8 ( G-I, L, O ). A greater number of atretic follicles were present within the pool of activated follicles at PND28 ( A, B ) PND16 ( D, E ) and PND8 ( G, H ) in Esr2-/- ovaries. Data shown as mean ± SE, n≥3. * P ≤ 0.05. Pd, Primordial follicle; Py, Primary follicle.

Journal: bioRxiv

Article Title: A gatekeeping role of ESR2 to maintain the primordial follicle reserve

doi: 10.1101/2020.02.06.937953

Figure Lengend Snippet: Increased activation of primordial follicles in Esr2-/- rat ovaries. Follicle counting in PND28 ( A-C, J, M ), PND16 ( D-F, K, N ), and PND8 ( G-I, L, O ) wildtype (WT) and Esr2-/- rats showed increased activation of primordial follicles in Esr2-/- ovaries. Primordial follicle activation was near two-fold starting on PND8 ( G-I, L, O ). A greater number of atretic follicles were present within the pool of activated follicles at PND28 ( A, B ) PND16 ( D, E ) and PND8 ( G, H ) in Esr2-/- ovaries. Data shown as mean ± SE, n≥3. * P ≤ 0.05. Pd, Primordial follicle; Py, Primary follicle.

Article Snippet: A selective ESR2 antagonist PHTPP ( ) or a selective ESR2 agonist DPN ( ) (Cayman Chemical, Ann Arbor, MI) was dissolved in DMSO (5µg/µl).

Techniques: Activation Assay

Premature ovarian senescence in Esr2-/- rats. The total number of follicles in PND8 ovaries were similar between wildtype (WT) and Esr2-/- rats; however, the number of primordial follicles were decreased in Esr2-/- ovaries ( A ). Follicle counts in 4wk, 12wk, and 24wk old WT and Esr2-/- rats revealed a sharp decline in the primordial follicle reserve in Esr2-/- ovaries ( B-H ). 24wk old Esr2-/- rats showed a significantly lower level of serum estradiol and AMH compared to WT ( I, J ). Data shown as mean± SE, n≥3 (follicle counting) and n≥6 (hormone assays), * P ≤ 0.05. Pd, Primordial follicle; Py, Primary follicle.

Journal: bioRxiv

Article Title: A gatekeeping role of ESR2 to maintain the primordial follicle reserve

doi: 10.1101/2020.02.06.937953

Figure Lengend Snippet: Premature ovarian senescence in Esr2-/- rats. The total number of follicles in PND8 ovaries were similar between wildtype (WT) and Esr2-/- rats; however, the number of primordial follicles were decreased in Esr2-/- ovaries ( A ). Follicle counts in 4wk, 12wk, and 24wk old WT and Esr2-/- rats revealed a sharp decline in the primordial follicle reserve in Esr2-/- ovaries ( B-H ). 24wk old Esr2-/- rats showed a significantly lower level of serum estradiol and AMH compared to WT ( I, J ). Data shown as mean± SE, n≥3 (follicle counting) and n≥6 (hormone assays), * P ≤ 0.05. Pd, Primordial follicle; Py, Primary follicle.

Article Snippet: A selective ESR2 antagonist PHTPP ( ) or a selective ESR2 agonist DPN ( ) (Cayman Chemical, Ann Arbor, MI) was dissolved in DMSO (5µg/µl).

Techniques:

Regulation of primordial follicle activation is ESR2-dependent. Deletion of the ESR2 DBD increased primordial follicle activation at PND28 ovaries ( A, B, G, I, J ), but this was not observed in ovaries from Esr1-/- rats ( C, G, K ). Administration of a selective ESR2 antagonist, PHTPP, into wildtype rats increased primordial follicle activation ( D, E, H, L, M ), whereas treatment with an ESR2 agonist, DPN, suppressed the activation ( F, H, N ). Data shown as mean ± SE, n ≥ 3. * P ≤ 0.05. Rel., Relative. Pd, Primordial follicle; Py, Primary follicle.

Journal: bioRxiv

Article Title: A gatekeeping role of ESR2 to maintain the primordial follicle reserve

doi: 10.1101/2020.02.06.937953

Figure Lengend Snippet: Regulation of primordial follicle activation is ESR2-dependent. Deletion of the ESR2 DBD increased primordial follicle activation at PND28 ovaries ( A, B, G, I, J ), but this was not observed in ovaries from Esr1-/- rats ( C, G, K ). Administration of a selective ESR2 antagonist, PHTPP, into wildtype rats increased primordial follicle activation ( D, E, H, L, M ), whereas treatment with an ESR2 agonist, DPN, suppressed the activation ( F, H, N ). Data shown as mean ± SE, n ≥ 3. * P ≤ 0.05. Rel., Relative. Pd, Primordial follicle; Py, Primary follicle.

Article Snippet: A selective ESR2 antagonist PHTPP ( ) or a selective ESR2 agonist DPN ( ) (Cayman Chemical, Ann Arbor, MI) was dissolved in DMSO (5µg/µl).

Techniques: Activation Assay

Activation of AKT, ERK, and mTOR signaling in Esr2-/- ovaries. Expression of ESR2 was detected in PND4, 6, and 8 rat ovaries using RT-qPCR ( A ) and western blotting ( B ). Primordial (Pd) ( C ), and primary (Py) ( D ) follicles were isolated from rat ovaries by digestion with liberase followed by size fractionation with strainers. RT-qPCR analysis demonstrated Esr2 expression in both Pd and Py follicles ( E ). Western blot analyses of PND8 ovaries and quantification of signal intensities demonstrated a significant increase in AKT ( F-I ) and ERK1/2 ( L-M ) activation. This was associated with increased activation of mTORC1 ( P-Q ) and its targets P70S6K ( R-S ) and RPS6 ( T-U ). But no difference was observed in PTEN ( J-K ) and pTSC2 ( N-O ) levels. Signal quantification data are presented as mean ± SEM. n ≥ 6. * P ≤ 0.05. Rel., Relative.

Journal: bioRxiv

Article Title: A gatekeeping role of ESR2 to maintain the primordial follicle reserve

doi: 10.1101/2020.02.06.937953

Figure Lengend Snippet: Activation of AKT, ERK, and mTOR signaling in Esr2-/- ovaries. Expression of ESR2 was detected in PND4, 6, and 8 rat ovaries using RT-qPCR ( A ) and western blotting ( B ). Primordial (Pd) ( C ), and primary (Py) ( D ) follicles were isolated from rat ovaries by digestion with liberase followed by size fractionation with strainers. RT-qPCR analysis demonstrated Esr2 expression in both Pd and Py follicles ( E ). Western blot analyses of PND8 ovaries and quantification of signal intensities demonstrated a significant increase in AKT ( F-I ) and ERK1/2 ( L-M ) activation. This was associated with increased activation of mTORC1 ( P-Q ) and its targets P70S6K ( R-S ) and RPS6 ( T-U ). But no difference was observed in PTEN ( J-K ) and pTSC2 ( N-O ) levels. Signal quantification data are presented as mean ± SEM. n ≥ 6. * P ≤ 0.05. Rel., Relative.

Article Snippet: A selective ESR2 antagonist PHTPP ( ) or a selective ESR2 agonist DPN ( ) (Cayman Chemical, Ann Arbor, MI) was dissolved in DMSO (5µg/µl).

Techniques: Activation Assay, Expressing, Quantitative RT-PCR, Western Blot, Isolation, Fractionation

Transcript levels of known activators of the AKT and mTOR pathways. RT-qPCR was performed on PND8 wildtype (WT) and Esr2-/- rat ovaries to analyze the expression of genes involved in activation of the AKT and mTOR pathways and activation of primordial follicles. While the expression of Kitlg ( A ), Kit ( B ), Igf1 ( C ), Bmp15 ( H ) and Gdf9 ( I ) was moderately upregulated in Esr2-/- rat ovaries, Adcyap1r1 ( F ), Bmp4 ( G ), Gata4 ( N ), and Npm2 ( O ) were highly upregulated. But no significant differences in the expression of Fgf2 ( D ), Ntrk2 ( E ), Amh ( J ), Bdnf ( K ), Nobox ( L ) and Sohlh1 ( M ) were observed between the WT and Esr2-/- rat ovaries. RT-qPCR data represent the mean ± SEM. n ≥ 8. * P ≤ 0.05. Rel., Relative.

Journal: bioRxiv

Article Title: A gatekeeping role of ESR2 to maintain the primordial follicle reserve

doi: 10.1101/2020.02.06.937953

Figure Lengend Snippet: Transcript levels of known activators of the AKT and mTOR pathways. RT-qPCR was performed on PND8 wildtype (WT) and Esr2-/- rat ovaries to analyze the expression of genes involved in activation of the AKT and mTOR pathways and activation of primordial follicles. While the expression of Kitlg ( A ), Kit ( B ), Igf1 ( C ), Bmp15 ( H ) and Gdf9 ( I ) was moderately upregulated in Esr2-/- rat ovaries, Adcyap1r1 ( F ), Bmp4 ( G ), Gata4 ( N ), and Npm2 ( O ) were highly upregulated. But no significant differences in the expression of Fgf2 ( D ), Ntrk2 ( E ), Amh ( J ), Bdnf ( K ), Nobox ( L ) and Sohlh1 ( M ) were observed between the WT and Esr2-/- rat ovaries. RT-qPCR data represent the mean ± SEM. n ≥ 8. * P ≤ 0.05. Rel., Relative.

Article Snippet: A selective ESR2 antagonist PHTPP ( ) or a selective ESR2 agonist DPN ( ) (Cayman Chemical, Ann Arbor, MI) was dissolved in DMSO (5µg/µl).

Techniques: Quantitative RT-PCR, Expressing, Activation Assay

ESR2 signaling in the regulation of primordial follicle activation. Loss of ESR2 leads to upregulation of both granulosa cell and oocyte derived factors that can activate the AKT, ERK and mTOR pathways. Increased levels of KITLG, KIT and IGF1, as well as NPM2, and ADCYAP1R1 can activate the AKT pathway followed by the mTOR pathway. Moreover, upregulation of NPM2 and BMP4 can activate the ERK pathway. Activated AKT, ERK and mTOR pathways in association with the transcriptional regulator GATA4 can promote the transition of primordial follicles to primary follicles in Esr2-/- ovaries.

Journal: bioRxiv

Article Title: A gatekeeping role of ESR2 to maintain the primordial follicle reserve

doi: 10.1101/2020.02.06.937953

Figure Lengend Snippet: ESR2 signaling in the regulation of primordial follicle activation. Loss of ESR2 leads to upregulation of both granulosa cell and oocyte derived factors that can activate the AKT, ERK and mTOR pathways. Increased levels of KITLG, KIT and IGF1, as well as NPM2, and ADCYAP1R1 can activate the AKT pathway followed by the mTOR pathway. Moreover, upregulation of NPM2 and BMP4 can activate the ERK pathway. Activated AKT, ERK and mTOR pathways in association with the transcriptional regulator GATA4 can promote the transition of primordial follicles to primary follicles in Esr2-/- ovaries.

Article Snippet: A selective ESR2 antagonist PHTPP ( ) or a selective ESR2 agonist DPN ( ) (Cayman Chemical, Ann Arbor, MI) was dissolved in DMSO (5µg/µl).

Techniques: Activation Assay, Derivative Assay